Anti-KDM4C / GASC1 / JMJD2C antibody (ab226480)
Key features and details
- Rabbit polyclonal to KDM4C / GASC1 / JMJD2C
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KDM4C / GASC1 / JMJD2C antibody -
Description
Rabbit polyclonal to KDM4C / GASC1 / JMJD2C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human KDM4C/ GASC1/ JMJD2C aa 475-525. The exact sequence is proprietary. (NP_055876.2).
Database link: Q9H3R0 -
Positive control
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226480 was affinity purified using an epitope specific to KDM4C / GASC1 / JMJD2C immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KDM4C / GASC1 / JMJD2C antibody (ab226480)All lanes : Anti-KDM4C / GASC1 / JMJD2C antibody (ab226480) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 120 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-KDM4C / GASC1 / JMJD2C antibody (ab226480)KDM4C / GASC1 / JMJD2C was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226480 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226480 at 1 µg/ml.
Lane 1: ab226480 (Lot 1) IP in HeLa whole cell lysate.
Lane 2: ab226480 (Lot 2) IP in HeLa whole cell lysate.
Lane 3: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
