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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Methylation

Anti-KDM2B antibody [6F6G11] (ab234082)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-KDM2B antibody [6F6G11] (ab234082)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [6F6G11] to KDM2B
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-KDM2B antibody [6F6G11]
    See all KDM2B primary antibodies
  • Description

    Mouse monoclonal [6F6G11] to KDM2B
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment corresponding to Human KDM2B aa 457-555. Expressed in E.coli.
    Database link: Q8NHM5

  • Positive control

    • WB: Human KDM2B (aa 457-555) recombinant protein; KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate; A549 cell lysate. Flow cyt: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    6F6G11
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Lysine demethylation

Images

  • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
    Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
    All lanes : Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate.
    Lane 2 : KDM2B (aa 457-555)-hIgGFc-transfected HEK-293 cell lysate.

    Predicted band size: 153 kDa

  • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
    Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
    Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution + Human KDM2B (aa 457-555) recombinant protein.

    Predicted band size: 153 kDa



    Expected recombinant MW: 36.8 kDa

  • Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)
    Flow Cytometry - Anti-KDM2B antibody [6F6G11] (ab234082)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling KDM2B using ab234082 at 1/200 dilution (green) compared to a negative control (red).

  • Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
    Western blot - Anti-KDM2B antibody [6F6G11] (ab234082)
    Anti-KDM2B antibody [6F6G11] (ab234082) at 1/100 dilution + A549 (human lung carcinoma cell line) cell lysate.

    Predicted band size: 153 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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