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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin E3 Enzymes RING Finger E3 Ligase

Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)

Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16312] to KCTD5 - BSA and Azide free
  • Suitable for: WB, ICC, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-KCTD5 antibody [EPR16312] - BSA and Azide free
    See all KCTD5 primary antibodies
  • Description

    Rabbit monoclonal [EPR16312] to KCTD5 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251193 is the carrier-free version of ab194825. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251193 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Clonality

    Monoclonal
  • Clone number

    EPR16312
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • RING Finger E3 Ligase

Images

  • Western blot - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    Western blot - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysates
    Lane 2 : Jurkat whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa



    This data was developed using ab194825, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    This data was developed using ab194825, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194825.
    Note: Both cytoplasm and nuclear staining on human kidney tissue. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Western blot - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    Western blot - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    All lanes : Anti-KCTD5 antibody [EPR16312] - C-terminal (ab194825) at 1/1000 dilution

    Lane 1 : C6 whole cell lysate
    Lane 2 : RAW 264.7 whole cell lysate
    Lane 3 : NIH/3T3 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 26 kDa



    This data was developed using ab194825, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    This data was developed using ab194825, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 was used as secondary. Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194825.
    Note: Both cytoplasm and nuclear staining on rat liver tissue. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    Immunocytochemistry - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    This data was developed using ab194825, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling KCTD5 with ab194825 at 1/100 dilution (3.8 μg/ml), followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Counterstained with Dapi (blue). Both cytoplasm and nuclear staining on A431 cell line is observed.
  • Flow Cytometry - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    Flow Cytometry - Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    This data was developed using ab194825, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Jurkat cells labeling KCTD5 with ab194825 at 1/70 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody (Blue). Rabbit monoclonal IgG was used as isotype control (Black).
  • Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)
    Anti-KCTD5 antibody [EPR16312] - BSA and Azide free (ab251193)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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