Anti-KCNN4 antibody (ab75956)
Key features and details
- Rabbit polyclonal to KCNN4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KCNN4 antibody
See all KCNN4 primary antibodies -
Description
Rabbit polyclonal to KCNN4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human KCNN4 aa 400 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab87266) -
Positive control
- This antibody gave a positive signal in the following human tissue lysates: Skin Placenta
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications PigApplication Abreviews Notes WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa).Target
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Function
Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T cells. The channel is blocked by clotrimazole and charybdotoxin but is insensitive to apamin. -
Tissue specificity
Widely expressed in non-excitable tissues. -
Sequence similarities
Belongs to the potassium channel KCNN family. KCa3.1/KCNN4 subfamily. -
Post-translational
modificationsPhosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3783 Human
- Entrez Gene: 397081 Pig
- Omim: 602754 Human
- SwissProt: O15554 Human
- Unigene: 10082 Human
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Alternative names
- DHS2 antibody
- hIKCa1 antibody
- hKCa4 antibody
see all
Images
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Western blot - Anti-KCNN4 antibody (ab75956)All lanes : Anti-KCNN4 antibody (ab75956) at 1 µg/ml
Lane 1 : Human skin tissue lysate - total protein (ab30166)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
The 50 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to KCNN4.
Datasheets and documents
References (1)
ab75956 has been referenced in 1 publication.
- Morales P et al. Contribution of the KCa3.1 channel-calmodulin interactions to the regulation of the KCa3.1 gating process. J Gen Physiol 142:37-60 (2013). WB . PubMed: 23797421
Images
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Western blot - Anti-KCNN4 antibody (ab75956)All lanes : Anti-KCNN4 antibody (ab75956) at 1 µg/ml
Lane 1 : Human skin tissue lysate - total protein (ab30166)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
The 50 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to KCNN4.
