Anti-KAT2B / PCAF antibody (ab12188)
Key features and details
- Rabbit polyclonal to KAT2B / PCAF
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KAT2B / PCAF antibody
See all KAT2B / PCAF primary antibodies -
Description
Rabbit polyclonal to KAT2B / PCAF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chicken
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Immunogen
Synthetic peptide:
EKFFFSKIKEAGLIDK
(with N-terminal added cysteine) conjugated to KLH, corresponding to the C-terminal amino acids 817-832 of Human PCAF. -
Positive control
- WB: HeLa, HEK293, Jurkat and Y79 cell lysates IP: Jurkat cells
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General notes
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody is affinity-purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KAT2B / PCAF antibody (ab12188)All lanes : Anti-KAT2B / PCAF antibody (ab12188) at 2 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate with KAT2B / PCAF immunizing peptide
Lane 3 : HepG2 cell lysate
Lane 4 : HepG2 cell lysate with KAT2B / PCAF immunizing peptide
Lane 5 : Jurkat cell lysate
Lane 6 : Jurkat cell lysate with KAT2B / PCAF immunizing peptide
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 93 kDaChemiluminescent substrate.
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Immunoprecipitation - Anti-KAT2B / PCAF antibody (ab12188)ab12188 was used to immunoprecipitate KAT2B / PCAF from an exctract of Jurkat cells. Detection antibody: Rabbit Anti-PCAF (C-terminal).
Lane 1: 5 μg/mL ab12188
Lane 2: Negative control: without antibody
Lane 3: 10 μg/mL ab12188
Lane 4: Negative control: without cell extract -
Western blot - Anti-KAT2B / PCAF antibody (ab12188)All lanes : Anti-KAT2B / PCAF antibody (ab12188) at 2 µg/ml
Lane 1 : Y79 cell lysates
Lane 2 : Y79 cell lysates with KAT2B / PCAF immunizing peptide
Secondary
All lanes : Goat Anti-Rabbit IgG-Peroxidase
Predicted band size: 93 kDachemiluminescent substrate.
