Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KAT2A / GCN5 antibody [EPR21146] - BSA and Azide free (ab231075)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21146] to KAT2A / GCN5 - BSA and Azide free
  • Suitable for: ChIP, WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KAT2A / GCN5 antibody [EPR21146] - BSA and Azide free
    See all KAT2A / GCN5 primary antibodies

  • Description

    Rabbit monoclonal [EPR21146] to KAT2A / GCN5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ChIP, WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa whole cell lysate and human fetal brain tissue lysate

  • General notes

    Ab231075 is the carrier-free version of ab217876. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab231075 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-KAT2A / GCN5 antibody [EPR21146] - BSA and Azide free (ab231075)
    Western blot - Anti-KAT2A / GCN5 antibody [EPR21146] - BSA and Azide free (ab231075)
    All lanes : Anti-KAT2A / GCN5 antibody [EPR21146] - ChIP Grade (ab217876) at 1/1000 dilution

    Lane 1 : Wild type HAP1 whole cell lysate
    Lane 2 : KAT2A / GCN5-knockout HAP1 whole cell lysate whole cell lysate
    Lane 3 : HEK-293T (uman epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 94 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST

    Exposure time: 3 minutes

    ab217876 was shown to specifically react with KAT2A in wild type HAP1 cells. No band was observed when KAT2A knockout samples were used. Wild-type and KAT2A knockout samples were subjected to SDS-PAGE. ab217876 and ab181602 (Human anti-GAPDH loading control) were incubated overnight at 4°C at a 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217876).

  • ChIP - Anti-KAT2A / GCN5 antibody [EPR21146] - BSA and Azide free (ab231075)
    ChIP - Anti-KAT2A / GCN5 antibody [EPR21146] - BSA and Azide free (ab231075)

    Chromatin was prepared from A549 treated with thapsigargin (1µM 12 hours) cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab217876 (red), and 20 µl of Protein A/G sepharose beads. 5 µg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

    Primers and probes are located in the first kb of the transcribed region.
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217876).

  • Immunoprecipitation - Anti-KAT2A / GCN5 antibody [EPR21146] - BSA and Azide free (ab231075)
    Immunoprecipitation - Anti-KAT2A / GCN5 antibody [EPR21146] - BSA and Azide free (ab231075)

    KAT2A / GCN5 was immunoprecipitated from 0.35mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab217876 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217876 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10ug (Input). 

    Lane 2: ab217876 IP in HeLa whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217876 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab217876).

  • Anti-KAT2A / GCN5 antibody [EPR21146] - BSA and Azide free (ab231075)
    Anti-KAT2A / GCN5 antibody [EPR21146] - BSA and Azide free (ab231075)

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