Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KAP1 antibody [EPR5249] - BSA and Azide free (ab247904)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5249] to KAP1 - BSA and Azide free
  • Suitable for: IP, ICC/IF, WB, Flow Cyt, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-KAP1 antibody [EPR5249] - BSA and Azide free
    See all KAP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5249] to KAP1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Although the immunogen is from a phosphor-peptide, this antibody detects phospho and non-phospho KAP1. Based on a peptide blocking experiment it has been found that the signal generated after non-phospho peptide blocking became much weaker, thus indicating that this antibody shows cross-reactivity with the non-phospho KAP1 at high level.

  • Tested applications

    Suitable for: IP, ICC/IF, WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: A431 cell lysate

  • General notes

    Ab247904 is the carrier-free version of ab109545. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247904 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-KAP1 antibody [EPR5249] - BSA and Azide free (ab247904)
    Immunoprecipitation - Anti-KAP1 antibody [EPR5249] - BSA and Azide free (ab247904)

    KAP1 was immunoprecipitated from 0.35 mg A431 (Human epidermoid carcinoma epithelial cell) cell lysate 10 µg with ab109545 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab109545 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: A431 (Human epidermoid carcinoma epithelial cell) cell lysate 10 µg

    Lane 2: ab109545 IP in A431 cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109545 in A431 cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 32 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109545).

  • Anti-KAP1 antibody [EPR5249] - BSA and Azide free (ab247904)
    Anti-KAP1 antibody [EPR5249] - BSA and Azide free (ab247904)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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