DNA demethylase (total) Activity Quantification Assay Kit (ab113472)
Key features and details
- Assay type: Enzyme activity
- Platform: Microplate reader
- Assay time: 3 hr
Overview
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Product name
DNA demethylase (total) Activity Quantification Assay Kit
See all DNA demethylase kits -
Assay type
Enzyme activity -
Assay time
3h 00m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Mammals
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Product overview
DNA methylation plays an essential role in biological processess through repression of transcription, while DNA demethylation is necessary, on the other hand, for epigenetic reprogramming of these genes. Active demethylation of DNA requires specific demethylase participation such as from MBD2.
DNA demethylase (total) Activity Quantification Assay Kit (ab113472) allows the user to measure total DNA demethylase activity/inhibition at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed for measuring total DNA demethylase activity/inhibition.
ab113472 is suitable for measuring DNA demethylase activity/inhibition from cultured cells/tissues or using purified enzyme/protein extracts.
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Notes
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components 48 tests 96 tests 10X Wash Buffer 1 x 11ml 1 x 22ml 8-Well Assay Strips (with Frame) 6 units 12 units Capture Antibody, 1000 µg/mL 1 x 5µl 1 x 8µl Demethylase Assay Buffer 1 x 3ml 1 x 6ml Demethylase Substrate 1 x 0.1ml 1 x 0.2ml Demethylation Standard, 10 µg/mL 1 x 20µl 1 x 40µl Detecting Antibody, 200 µg/mL 1 x 10µl 1 x 20µl Developing Solution 1 x 5ml 1 x 10ml Stop Solution 1 x 3ml 1 x 6ml Substrate Binding Solution 1 x 5ml 1 x 10ml -
Research areas
Images
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Functional Studies - DNA demethylase (total) Activity Quantification Assay Kit (ab113472)
Nuclear extracts were prepared from MCF-7 cells using the Nuclear Extraction Kit (ab113474) and total DNA demethylase activity was measured using ab113472.
