Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free
See all JunD+c-Jun primary antibodies
Description

Rabbit monoclonal [EPR16586] to JunD+c-Jun (phospho S73) - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Dot blot, WB, IP, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
ab250081 is the carrier-free version of ab178858.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16586
Isotype

IgG
Research areas

Western blot - Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)

All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate
Lane 2 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 40,45,48 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab178858, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100).
Dot Blot - Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)

This data was developed using ab178858, the same antibody clone in a different buffer formulation.
Dot blot analysis of JunD (phospho S100) + c-Jun (phospho S73) peptide (Lane 1) and non-phospho peptide (Lane 2) labeled using ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.Exposure time: 3 minutes.
Western blot - Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)

Lanes 1 & 4-5 : Anti-c-Jun antibody [E254] - ChIP Grade (ab32137)
Lanes 2-3 : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution

Lanes 1 & 3 & 5 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lanes 2 & 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 40,45 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab178858, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)

All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution

Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 250 ng/ml Anisomycin for 30 minutes, whole cell lysate treated with Alkaline Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 40,45 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab178858, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)

All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/10000 dilution

Lane 1 : NIH/3T3 (Mouse embryo fibroblast cell line) treated with 250 ng/ml Anisomycin for 30 minutes whole cell lysate
Lane 2 : Untreated NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG,(H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 40,45 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

This data was developed using ab178858, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100).
Western blot - Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)

All lanes : Anti-JunD (phospho S100) + c-Jun (phospho S73) antibody [EPR16586] (ab178858) at 1/1000 dilution

Lane 1 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 40,45 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

This data was developed using ab178858, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.

Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)

This data was developed using ab178858, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling JunD (phospho S100) + c-Jun (phospho S73) with ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on lymphocytes and endothelial cells of Human tonsil is observed. Counter stained with Hematoxylin. Negative Control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)

This data was developed using ab178858, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling JunD (phospho S100) + c-Jun (phospho S73) with ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on spermatogoniums and Leydig cells of mouse testis is observed. Counter stained with Hematoxylin. Negative Control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)

This data was developed using ab178858, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling JunD (phospho S100) + c-Jun (phospho S73) with ab178858 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on bile duct epithelial cells while no staining on hepatocytes of rat liver is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)

This data was developed using ab178858, the same antibody clone in a different buffer formulation.JunD (phospho S100) + c-Jun (phospho S73) were immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma), treated with 250ng/ml Anisomycin for 30 minutes, whole cell extract with ab178858 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab178858 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa, treated with 250ng/ml Anisomycin for 30 minutes, whole cell extract
Lane 2: PBS.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds. Per the blast, ab178858 could recognize JunD (phospho Ser100) with 100% homology. Multi-bands are due to c-Jun (phospho Ser73) & JunD (phospho Ser100)
Anti-JunD+c-Jun (phospho S73) antibody [EPR16586] - BSA and Azide free (ab250081)