Anti-JAB1 antibody (ab12323)
Key features and details
- Rabbit polyclonal to JAB1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-JAB1 antibody
See all JAB1 primary antibodies -
Description
Rabbit polyclonal to JAB1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Xenopus tropicalis
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Immunogen
Immunogen was a synthetic peptide, which represented a c-terminal domain of human JAB1 (LocusLink ID 10987).
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General notes
Centrifuge tube to remove product from lid.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-JAB1 antibody (ab12323) This image is courtesy of an anonymous AbreviewAll lanes : Anti-JAB1 antibody (ab12323) at 1/2500 dilution
Lane 1 : HEK293 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lane 3 : U2OS whole cell lysate
Lane 4 : Mouse embryonic fibroblast whole cell lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JAB1 antibody (ab12323)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma (left) and mouse teratoma (right) tissues labelling JAB1 with ab12323 at 1/500 (2µg/ml). Detection: DAB. -
Western blot - Anti-JAB1 antibody (ab12323)Detection of Human CSN5 by Western Blot.
Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody: Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure. Detection of Human CSN5 by Western Blot. Samples: Whole cell lysate (50 mcg E; 25 mcg T) from HEK 293T cells that were mock transfected (E) or transfected with a CSN5 expression construct (T). Antibody: Affinity purified rabbit anti-CSN5 antibody BL1062 used at the indicated concentrations. Detection: Chemiluminescence with a 10 second exposure.
