Anti-ISG15 antibody [EPR3446] - BSA and Azide free (ab248489)
![Anti-ISG15 antibody [EPR3446] - BSA and Azide free (ab248489)](https://www.abcam.com/ps/products/248/ab248489/Images/ab248489-410675-anti-isg15-antibody-epr3446-bsa-and-azide-free-western-blot.jpg "Anti-ISG15 antibody [EPR3446] - BSA and Azide free (ab248489)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3446] to ISG15 - BSA and Azide free
- Suitable for: ICC, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ISG15 antibody [EPR3446] - BSA and Azide free
See all ISG15 primary antibodies -
Description
Rabbit monoclonal [EPR3446] to ISG15 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248489 is the carrier-free version of ab133346 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248489 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR3446 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ISG15 antibody [EPR3446] - BSA and Azide free (ab248489)
This data was developed using ab133346, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: HAP1 IFN treated wild-type HAP1 whole cell lysate (20 µg)
Lane 3: ISG15 (KO) whole cell lysate (20 µg)
Lane 4: ISG15 (KO) IFN alpha treated whole cell lysate (20 µg)
Lane 5: Daudi whole cell lysate (20 µg)
Lanes 1 - 5: Merged signal (red and green). Green - ab133346 observed at 18 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab133346 was shown to specifically react with HAP1 IFN treated when HAP1 IFN treated knockout samples were used. Wild-type and HAP1 IFN treated knockout samples were subjected to SDS-PAGE. ab133346 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/30000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ISG15 antibody [EPR3446] - BSA and Azide free (ab248489)](https://www.abcam.com/ps/products/248/ab248489/Images/ab248489-407894-anti-isg15-antibody-epr3446-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ISG15 antibody [EPR3446] - BSA and Azide free (ab248489)All lanes : Anti-ISG15 antibody [EPR3446] (ab133346) at 1/2000 dilution
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 10 ng/ml IFN- a1 for 16 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab133346, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunocytochemistry - Anti-ISG15 antibody [EPR3446] - BSA and Azide free (ab248489)](https://www.abcam.com/ps/products/248/ab248489/Images/ab248489-1-anti-isg15-antibody-epr3446-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-ISG15 antibody [EPR3446] - BSA and Azide free (ab248489) -
![Anti-ISG15 antibody [EPR3446] - BSA and Azide free (ab248489)](https://www.abcam.com/ps/products/248/ab248489/Images/ab248489-4-benefits-of-recombinant-antibodies.png)
Anti-ISG15 antibody [EPR3446] - BSA and Azide free (ab248489)
