Anti-Iroquois homeobox protein 3/IRX3 antibody (ab25703)
Key features and details
- Rabbit polyclonal to Iroquois homeobox protein 3/IRX3
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Iroquois homeobox protein 3/IRX3 antibody
See all Iroquois homeobox protein 3/IRX3 primary antibodies -
Description
Rabbit polyclonal to Iroquois homeobox protein 3/IRX3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
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General notes
This product was previously labelled as Iroquois homeobox protein 3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications ChickenHumanXenopus laevisZebrafishApplication Abreviews Notes WB Use a concentration of 2 µg/ml. Detects a band of approximately 52, 55 kDa (predicted molecular weight: 52 kDa).Notes WB
Use a concentration of 2 µg/ml. Detects a band of approximately 52, 55 kDa (predicted molecular weight: 52 kDa).Target
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Function
Transcription factor. Involved in SHH-dependent neural patterning. Together with NKX2-2 and NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class I proteins of neuronal progenitor factors, which are repressed by SHH signals. Involved in the transcriptional repression of MNX1 in non-motor neuron cells. -
Sequence similarities
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 408181 Chicken
- Entrez Gene: 79191 Human
- Entrez Gene: 16373 Mouse
- Entrez Gene: 399365 Xenopus laevis
- Entrez Gene: 30520 Zebrafish
- Omim: 612985 Human
- SwissProt: P78415 Human
- SwissProt: P81067 Mouse
see all -
Alternative names
- AI894186 antibody
- Homeodomain protein IRXB1 antibody
- Iroquois class homeodomain protein IRX 1 antibody
see all
Images
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Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody (ab25703)All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody (ab25703) at 2 µg/ml
Lane 1 : Mouse Fetus (14 Day Old) Tissue Lysate
Lane 2 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52,55 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
References (4)
ab25703 has been referenced in 4 publications.
- Zou Y et al. IRX3 Promotes the Browning of White Adipocytes and Its Rare Variants are Associated with Human Obesity Risk. EBioMedicine 24:64-75 (2017). PubMed: 28988979
- Wang P et al. Downregulation of miR-377 contributes to IRX3 deregulation in hepatocellular carcinoma. Oncol Rep 36:247-52 (2016). PubMed: 27222047
- Shekhar A et al. Transcription factor ETV1 is essential for rapid conduction in the heart. J Clin Invest 126:4444-4459 (2016). IHC-Fr ; Mouse . PubMed: 27775552
- Gaborit N et al. Cooperative and antagonistic roles for Irx3 and Irx5 in cardiac morphogenesis and postnatal physiology. Development 139:4007-19 (2012). ChIP ; Mouse . PubMed: 22992950
Images
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Western blot - Anti-Iroquois homeobox protein 3/IRX3 antibody (ab25703)All lanes : Anti-Iroquois homeobox protein 3/IRX3 antibody (ab25703) at 2 µg/ml
Lane 1 : Mouse Fetus (14 Day Old) Tissue Lysate
Lane 2 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52,55 kDa why is the actual band size different from the predicted?
