Anti-C14orf166 antibody [EPR15799] (ab188326)
![Anti-C14orf166 antibody [EPR15799] (ab188326)](https://www.abcam.com/ps/products/188/ab188326/Images/ab188326-221855-anti-c14orf166-antibody-epr15799-western-blot.jpg "Anti-C14orf166 antibody [EPR15799] (ab188326)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15799] to C14orf166
- Suitable for: ICC/IF, IP, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-C14orf166 antibody [EPR15799]
See all C14orf166 primary antibodies -
Description
Rabbit monoclonal [EPR15799] to C14orf166 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB RatHuman
-
Immunogen
This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- U87-MG , Ramos, 293T, Jurkat, Raw264.7, C6, PC12 and NIH3T3 cell lysates; Human fetal kidney and astrocytoma tissues; U87-MG cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15799 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-C14orf166 antibody [EPR15799] (ab188326)All lanes : Anti-C14orf166 antibody [EPR15799] (ab188326) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
-
![Immunocytochemistry/ Immunofluorescence - Anti-C14orf166 antibody [EPR15799] (ab188326)](https://www.abcam.com/ps/products/188/ab188326/Images/ab188326-221863-anti-c14orf166-antibody-epr15799-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C14orf166 antibody [EPR15799] (ab188326)Immunofluorescence analysis of U87-MG cells labeling C14orf166 using ab188326 at 1/100 dilution. A goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] (ab188326)](https://www.abcam.com/ps/products/188/ab188326/Images/ab188326-221862-anti-c14orf166-antibody-epr15799-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] (ab188326)Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling C14orf166 using ab188326 at 1/100 dilution. A ready to use HRP polymer for rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] (ab188326)](https://www.abcam.com/ps/products/188/ab188326/Images/ab188326-221859-anti-c14orf166-antibody-epr15799-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] (ab188326)Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue labeling C14orf166 using ab188326 at 1/100 dilution. A ready to use HRP polymer for rabbit IgG (prediluted) was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunoprecipitation - Anti-C14orf166 antibody [EPR15799] (ab188326)](https://www.abcam.com/ps/products/188/ab188326/Images/ab188326-221857-anti-c14orf166-antibody-epr15799-immunoprecipitation.jpg)
Immunoprecipitation - Anti-C14orf166 antibody [EPR15799] (ab188326)Immunoprecipitation analysis of 293T cell lysate labeling C14orf166 using ab188326 at 1/50 dilution (Lane 1). An anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of 293T cell lysate.
-
![Western blot - Anti-C14orf166 antibody [EPR15799] (ab188326)](https://www.abcam.com/ps/products/188/ab188326/Images/ab188326-221854-anti-c14orf166-antibody-epr15799-western-blot.jpg)
Western blot - Anti-C14orf166 antibody [EPR15799] (ab188326)All lanes : Anti-C14orf166 antibody [EPR15799] (ab188326) at 1/10000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
-
![Anti-C14orf166 antibody [EPR15799] (ab188326)](https://www.abcam.com/ps/products/188/ab188326/Images/ab188326-7-benefits-of-recombinant-antibodies.png)
Anti-C14orf166 antibody [EPR15799] (ab188326)
