Anti-IRAKM antibody [Y278] (ab32394)
![Anti-IRAKM antibody [Y278] (ab32394)](https://www.abcam.com/ps/products/32/ab32394/Images/ab32394-267298-anti-irakm-antibody-y278-western-blot.jpg "Anti-IRAKM antibody [Y278] (ab32394)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y278] to IRAKM
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-IRAKM antibody [Y278]
See all IRAKM primary antibodies -
Description
Rabbit monoclonal [Y278] to IRAKM -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human IRAKM aa 550 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9Y616 -
Positive control
- A431 cell lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
Y278 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRAKM antibody [Y278] (ab32394)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IRAKM knockout HAP1 cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab32394 observed at 69 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32394 was shown to specifically react with IRAKM when IRAKM knockout samples were used. Wild-type and IRAKM knockout samples were subjected to SDS-PAGE. ab32394 and ab8245 (loading control to GAPDH) diluted to 1/5000 and 1/10000 respectively were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-IRAKM antibody [Y278] (ab32394)](https://www.abcam.com/ps/products/32/ab32394/Images/ab32394-44695-anti-irakm-antibody-y278-western-blot.jpg)
Western blot - Anti-IRAKM antibody [Y278] (ab32394)Anti-IRAKM antibody [Y278] (ab32394) at 1/20000 dilution + A431 cell lysate
Predicted band size: 68 kDa
Observed band size: 68 kDa
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![Anti-IRAKM antibody [Y278] (ab32394)](https://www.abcam.com/ps/products/32/ab32394/Images/ab32394-3-benefits-of-recombinant-antibodies.png)
Anti-IRAKM antibody [Y278] (ab32394)
