Anti-IRAKM antibody (ab8116)
Key features and details
- Rabbit polyclonal to IRAKM
- Suitable for: ICC, IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-IRAKM antibody
See all IRAKM primary antibodies -
Description
Rabbit polyclonal to IRAKM -
Host species
Rabbit -
Specificity
Anti-IRAK-M has no cross response to IRAK or IRAK2. -
Tested applications
Suitable for: ICC, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide:
CSSKFSWDEYEQYKKE
, corresponding to C terminal amino acids 581-596 of Human IRAKM (Peptide available as ab6231.) -
General notes
Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified IgG prepared by ion exchange chromatography. IRAK-M Antibody is affinity chromatography purified via peptide column. -
Primary antibody notes
Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRAKM antibody (ab8116)All lanes : Anti-IRAKM antibody (ab8116) at 1 µg/ml
Lane 1 : Mouse spleen tissue lysates
Lane 2 : Rat liver tissue lysate
Observed band size: 68 kDa why is the actual band size different from the predicted?
Western blot analysis of IRAKM in mouse spleen (M) and rat liver (R) tissue lysates with anti-IRAKM at 1µ -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of A20 cells staining IRAKM with ab8116 at 20 µg/ml -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAKM antibody (ab8116)ab8116 at 2µg/ml staining IRAKM in rat liver cells by IHC
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Immunocytochemistry - Anti-IRAKM antibody (ab8116) Image from Lyn-Kew K et al, PLoS One. 2010 Jun 16;5(6):e11145, Fig 4.ab8116 staining IRAKM in murine lung macrophages by Immunocytochemistry/ Immunofluorescence.<.br>
The cells were permeablized with a 1:1 mixture of methanol and acetone. After washing with PBS, ab8116 was applied and the cells incubated for one hour. After washing, antibodies against the primary antibody labeled with FITC were applied to the cells and incubated for one hour. The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope. -
Immunocytochemistry - Anti-IRAKM antibody (ab8116)Immunofluorescence of IRAK-M in Rat Liver cells using ab8116 at 10 ug/ml.
