Anti-Involucrin antibody - N-terminal (ab227530)
Key features and details
- Rabbit polyclonal to Involucrin - N-terminal
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Involucrin antibody - N-terminal
See all Involucrin primary antibodies -
Description
Rabbit polyclonal to Involucrin - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Involucrin (N terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: P07476 -
Positive control
- WB: Involucrin transfected HEK-293T whole cell extract. Jurkat, Raji and NCI-H929 whole cell extract. ICC/IF: A431 cells. IHC-P: H661 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Involucrin antibody - N-terminal (ab227530)
Paraffin-embedded H661 xenograft stained for Involucrin with ab227530 (1/250 dilution) in immunohistochemical analysis.
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Western blot - Anti-Involucrin antibody - N-terminal (ab227530)All lanes : Anti-Involucrin antibody - N-terminal (ab227530) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extracts
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extracts
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 68 kDa7.5% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-Involucrin antibody - N-terminal (ab227530)A431 (human epidermoid carcinoma cell line) cells stained for Involucrin (green) using ab227530 (1/200 dilution) in ICC/IF. Cells were fixed in methanol.
Counterstain: Hoechst 33342 (blue).
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Western blot - Anti-Involucrin antibody - N-terminal (ab227530)All lanes : Anti-Involucrin antibody - N-terminal (ab227530) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : Involucrin-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 68 kDa7.5% SDS-PAGE
