Anti-INO80 antibody (ab105451)
Key features and details
- Rabbit polyclonal to INO80
- Suitable for: ICC/IF, IHC-Fr, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-INO80 antibody
See all INO80 primary antibodies -
Description
Rabbit polyclonal to INO80 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Dog, Pig, Macaque monkey, Gorilla
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Immunogen
Synthetic peptide corresponding to Human INO80 aa 1500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab123818) -
Positive control
- This antibody gave a positive signal in human brain tissue lysate and HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml.IHC-Fr (1) Use at an assay dependent concentration.WB Use a concentration of 1 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).Notes ICC/IF
Use a concentration of 1 µg/ml.IHC-Fr
Use at an assay dependent concentration.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).Target
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Function
DNA helicase and probable main scaffold component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair; according to PubMed:20687897 the contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Recruited by YY1 to YY1-activated genes, where it acts as an essential coactivator. Binds DNA. In vitro, has double stranded DNA-dependent ATPase activity. Involved in UV-damage excision repair, DNA replication and chromosome segregation during normal cell division cycle. -
Tissue specificity
According to PubMed:10574462, widely expressed. According to PubMed:16298340, specifically expressed in brain, liver and pancreas. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
Contains 1 DBINO domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Domain
The DBINO region is involved in binding to DNA. -
Cellular localization
Nucleus. Colocalizes with PCNA at replication forks during S-phase. Recruited to DNA damage sites in a ACTR8-dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 505992 Cow
- Entrez Gene: 478262 Dog
- Entrez Gene: 54617 Human
- Entrez Gene: 68142 Mouse
- Entrez Gene: 100517567 Pig
- Omim: 610169 Human
- SwissProt: Q9ULG1 Human
- SwissProt: Q6ZPV2 Mouse
see all -
Alternative names
- DNA helicase INO80 antibody
- hINO80 antibody
- homolog of yeast INO80 antibody
see all
Images
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Western blot - Anti-INO80 antibody (ab105451)All lanes : Anti-INO80 antibody (ab105451) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 177 kDa
Observed band size: 177 kDa
Additional bands at: 32 kDa, 64 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-INO80 antibody (ab105451)ICC/IF image of ab105451 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105451, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa, Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2, MCF7 cells at 1µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab105451 has been referenced in 6 publications.
- Cerase A et al. Chd8 regulates X chromosome inactivation in mouse through fine-tuning control of Xist expression. Commun Biol 4:485 (2021). PubMed: 33859315
- Seo HR et al. CHIP and BAP1 Act in Concert to Regulate INO80 Ubiquitination and Stability for DNA Replication. Mol Cells 44:101-115 (2021). PubMed: 33658435
- Wang Y et al. LncRNA HAND2-AS1 promotes liver cancer stem cell self-renewal via BMP signaling. EMBO J 38:e101110 (2019). PubMed: 31334575
- Rhee S et al. Endothelial deletion of Ino80 disrupts coronary angiogenesis and causes congenital heart disease. Nat Commun 9:368 (2018). PubMed: 29371594
- Runge JS et al. Identification of Two Distinct Classes of the Human INO80 Complex Genome-Wide. G3 (Bethesda) 8:1095-1102 (2018). PubMed: 29432129
- Serber DW et al. The Mouse INO80 Chromatin-Remodeling Complex Is an Essential Meiotic Factor for Spermatogenesis. Biol Reprod 94:8 (2016). ICC/IF ; Mouse . PubMed: 26607718
Images
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Western blot - Anti-INO80 antibody (ab105451)All lanes : Anti-INO80 antibody (ab105451) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 177 kDa
Observed band size: 177 kDa
Additional bands at: 32 kDa, 64 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-INO80 antibody (ab105451)ICC/IF image of ab105451 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105451, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa, Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2, MCF7 cells at 1µg/ml.
