Anti-INO80C antibody (ab151046)
Key features and details
- Rabbit polyclonal to INO80C
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-INO80C antibody -
Description
Rabbit polyclonal to INO80C -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment, corresponding to a sequence from Human INO80C.
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Positive control
- ICC: U-2 OS whole cells; IHC-P: Human kidney, testits and skeletal muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-INO80C antibody (ab151046)All lanes : Anti-INO80C antibody (ab151046) at 4 µg/ml
Lane 1 : Control (only vector transfected) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : INO80C over-expression (co-expressed with C-terminal myc-DDk tag) HEK-293T whole cell lysate
Predicted band size: 21 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-INO80C antibody (ab151046)Immunofluorescence analysis of U-2 OS (human bone osteosarcoma epithelial cell line) whole cells labeling INO80C at the nucleoli fibrilar center with ab151046 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human kidney tissue labeling INO80C of tubules cells with ab151046 at 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot - Anti-INO80C antibody (ab151046)Anti-INO80C antibody (ab151046) at 4 µg/ml + HEL (human bone marrow erythroleukemia cell line) whole cell lysate
Predicted band size: 21 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human testis tissue labeling INO80C at the nuclues of seminiferous ducts with ab151046 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INO80C antibody (ab151046)Immunohistochemistry analysis of human skeletal muscle tissue labeling INO80C of myocytes with ab151046 at a 1/200 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-INO80C antibody (ab151046) This image is courtesy of an anonymous Abreviewab151046 staining INO80C in Mouse paraffin-fixed tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/100 in Goat serum) for 24 hours at 4°C. An Alexa Fluor® 555-conjugated anti-rabbit IgG polyclonal (1/125) was used as the secondary antibody. Red - INO80C, Blue (DAPI) - nuclei.