Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Inhibin alpha antibody [PO12/8] (ab273454)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [PO12/8] to Inhibin alpha
  • Suitable for: WB, ELISA, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Inhibin alpha antibody [PO12/8]
    See all Inhibin alpha primary antibodies

  • Description

    Mouse monoclonal [PO12/8] to Inhibin alpha

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis and placenta tissue lysates. IHC-P: Human testis and ovary tissue. ELISA: Human Inhibin alpha.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59.94% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    PO12/8

  • Isotype

    IgG2a

  • Research areas

Images

  • Western blot - Anti-Inhibin alpha antibody [PO12/8] (ab273454)
    Western blot - Anti-Inhibin alpha antibody [PO12/8] (ab273454)
    All lanes : Anti-Inhibin alpha antibody [PO12/8] (ab273454) at 1/1000 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : Human placenta tissue lysate
    Lane 3 : Human tonsil tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    Negative control: Human tonsil.
    The molecular weight observed is consistent with what has been described in the literature (PMID:31658054).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 180 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] (ab273454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] (ab273454)

    Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling Inhibin alpha with ab273454 at 1/1000 (0.937 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplamic staining on the theca interna of the human ovary. The section was incubated with ab273454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • ELISA - Anti-Inhibin alpha antibody [PO12/8] (ab273454)
    ELISA - Anti-Inhibin alpha antibody [PO12/8] (ab273454)

    ab273454 used at 0-2000 ng/ml.

    Antigen (Human Inhibin alpha) used at 1000 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] (ab273454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] (ab273454)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Inhibin alpha with ab273454 at 1/1000 (0.937 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplamic staining on human testis. The section was incubated with ab273454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Inhibin alpha antibody [PO12/8] (ab273454)
    Anti-Inhibin alpha antibody [PO12/8] (ab273454)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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