Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Inhibin alpha antibody [PO12/8] (ab273454)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [PO12/8] to Inhibin alpha
Suitable for: WB, ELISA, IHC-P
Reacts with: Human, Recombinant fragment

Product name

Anti-Inhibin alpha antibody [PO12/8]
See all Inhibin alpha primary antibodies
Description

Mouse monoclonal [PO12/8] to Inhibin alpha
Host species

Mouse

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human testis and placenta tissue lysates. IHC-P: Human testis and ovary tissue. ELISA: Human Inhibin alpha.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59.94% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

PO12/8
Isotype

IgG2a
Research areas

Western blot - Anti-Inhibin alpha antibody [PO12/8] (ab273454)

All lanes : Anti-Inhibin alpha antibody [PO12/8] (ab273454) at 1/1000 dilution

Lane 1 : Human testis tissue lysate
Lane 2 : Human placenta tissue lysate
Lane 3 : Human tonsil tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Predicted band size: 40 kDa
Observed band size: 40 kDa

Negative control: Human tonsil.
The molecular weight observed is consistent with what has been described in the literature (PMID:31658054).

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: 180 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] (ab273454)

Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling Inhibin alpha with ab273454 at 1/1000 (0.937 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplamic staining on the theca interna of the human ovary. The section was incubated with ab273454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
ELISA - Anti-Inhibin alpha antibody [PO12/8] (ab273454)

ab273454 used at 0-2000 ng/ml.

Antigen (Human Inhibin alpha) used at 1000 ng/ml.

Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Inhibin alpha antibody [PO12/8] (ab273454)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling Inhibin alpha with ab273454 at 1/1000 (0.937 µg/ml) dilution followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplamic staining on human testis. The section was incubated with ab273454 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Anti-Inhibin alpha antibody [PO12/8] (ab273454)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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