Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free (ab272699)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23484-151] to IL-9 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IP, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free
    See all IL-9 primary antibodies

  • Description

    Rabbit monoclonal [EPR23484-151] to IL-9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IP, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged mouse IL9 recombinant protein, Mouse and rat spleen, thymus lysates. ICC/IF: 293T cell. Flow Cyt: 293T cell. IP: Mouse spleen lysate.

  • General notes

    ab272699 is the carrier-free version of ab227037. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free (ab272699)
    Immunocytochemistry/ Immunofluorescence - Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free (ab272699)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing strong membranous and cytoplasmic staining in 293T cells transfected with HA tagged mouse IL9 construct. anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227037).

  • Flow Cytometry - Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free (ab272699)
    Flow Cytometry - Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free (ab272699)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution (0.1µg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227037).

  • Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free (ab272699)
    Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free (ab272699)

    IL-9 was immunoprecipitated from 0.35 mg mouse spleen tissue lysate 10µg with ab227037 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab227037 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse spleen tissue lysate 10µg

    Lane 2: ab227037 IP in mouse spleen tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227037 in mouse spleen tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This blot was developed using a higher sensitivity ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227037).

  • Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free (ab272699)
    Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free (ab272699)

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