Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22994-255] to IL-8 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free
    See all IL-8 primary antibodies

  • Description

    Rabbit monoclonal [EPR22994-255] to IL-8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U-87 MG treated with 1µM Thapsigargin for 24 hours, whole cell lysate; Wild-type PC-3 LPS-treated (2 µg/ml, 6 h) with Brefeldin A (ab120299) (5 µg/ml, 5 h) cell lysate; His-tagged human recombiant IL-8 protein (aa21-99).

  • General notes

    ab275255 is the carrier-free version of ab235584. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275255 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)
    Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)
    All lanes : Anti-IL-8 antibody [EPR22994-255] (ab235584) at 1/1000 dilution

    Lane 1 : Wild-type PC-3 Brefeldin A (ab120299)-treated (5 μg/ml, 5 h) cell lysate
    Lane 2 : Wild-type PC-3 LPS-treated (2 μg/ml, 6 h) with Brefeldin A (ab120299) (5 μg/ml, 5 h) cell lysate
    Lane 3 : CXCL8 knockout PC-3 Brefeldin A (ab120299)-treated (5 μg/ml, 5 h) cell lysate
    Lane 4 : CXCL8 knockout PC-3 LPS-treated (2 μg/ml, 6 h) with Brefeldin A (ab120299) (5 μg/ml, 5 h) cell lysate
    Lane 5 : A431 cell lysate
    Lane 6 : HCT116 cell lysate

    Lysates/proteins at 30 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 11 kDa
    Observed band size: 10 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab235584).

    Lanes 1 - 6: Merged signal (red and green). Green - ab235584 observed at 10 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab235584 was shown to react with IL-8 in wild-type PC-3 cells in Western blot with loss of signal observed in CXCL8 knockout sample. Wild-type PC-3 and CXCL8 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab235584 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)
    Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)
    Anti-IL-8 antibody [EPR22994-255] (ab235584) at 1/1000 dilution + His-tagged human recombiant IL-8 protein (aa21-99) 10 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa



    This data was developed using ab235584, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 minutes

  • Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)
    Western blot - Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)
    All lanes : Anti-IL-8 antibody [EPR22994-255] (ab235584) at 1/1000 dilution

    Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
    Lane 2 : U-87 MG treated with 1uM Thapsigargin for 24 hours, whole cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lane 2 : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 11 kDa



    This data was developed using ab235584, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 3 minutes

  • Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)
    Anti-IL-8 antibody [EPR22994-255] - BSA and Azide free (ab275255)

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