Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)
Key features and details
- Rabbit polyclonal to Calnexin - ER membrane marker - ER Marker
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Calnexin - ER membrane marker antibody - ER Marker
See all Calnexin primary antibodies -
Description
Rabbit polyclonal to Calnexin - ER membrane marker - ER Marker -
Host species
Rabbit -
Specificity
Weak reactivity with Chicken, Fruitfly (Drosophila melanogaster) and Xenopus laevis. -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide:
(C)EEDEILNRSPRNRKPRRE
conjugated to KLH, corresponding to C terminal amino acids 575-592 of Dog Calnexin. This sequence is identical to mouse, human and rat calnexin over these residues. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)
Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed 2% Formaldehyde for 20 min at RT. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)Immunohistochemistry analysis using ab13504 at 1/100 for 1 hour at RT in mouse backskin. Fixed by Bouin’s Fixative Solution. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1/50 for 1 hour at RT.
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Western blot - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)All lanes : Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504) at 1/2000 dilution
All lanes : Mixture of rat tissue lysates
Predicted band size: 90 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/100 for 12 hours at 4°C in HaCaT cells. Fixed by Cold 100% methanol at -20C for 10 minutes. Secondary Antibody: FITC Goat Anti-Rabbit at 1/50 for 1-2 hours at RT in dark. /
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Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Heat Shocked at 42°C for 1h.
