Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24322-143] to IL-6R - BSA and Azide free
  • Suitable for: ICC, IHC-P, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free
    See all IL-6R primary antibodies

  • Description

    Rabbit monoclonal [EPR24322-143] to IL-6R - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, IP, WBmore details
    Unsuitable for: Flow Cyt (Intra)

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U266B1 lysates. IHC-P: Human placenta, tonsil, thyroid carcinoma tissues. ICC: U266B1 cells. IP: U266B1 cells.

  • General notes

    ab280573 is the carrier-free version of ab271042. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab280573 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)
    Western blot - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)
    All lanes : Anti-IL-6R antibody [EPR24322-143] (ab271042) at 1/1000 dilution

    Lane 1 : U266B1 (human multiple myeloma B lymphocyte) whole cell lysate
    Lane 2 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 52 kDa
    Observed band size: 70-80 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab271042, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The observed MW is consistent with what has been described in the literature (PMID: 28276471, 21282511).

    Negative control: K-562 (PMID: 8300137)

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 37 seconds

  • Immunocytochemistry - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)
    Immunocytochemistry - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)

    This data was developed using ab271042, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U266B1 cells labelling IL-6R with ab271042 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U266B1 cells. Negative control: K-562 (PMID: 8300137) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)

    This data was developed using ab271042, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labelling IL-6R with ab271042 at 1/2000 dilution (0.255 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cyoplasmics staining on human thyroid carcinoma. The section was incubated with ab271042 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)

    This data was developed using ab271042, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IL-6R with ab271042 at 1/2000 dilution (0.255 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cyoplasmics staining on human tonsil. The section was incubated with ab271042 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)

    This data was developed using ab271042, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling IL-6R with ab271042 at 1/2000 dilution (0.255 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cyoplasmics staining on human placenta (PMID: 25658637). The section was incubated with ab271042 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Immunoprecipitation - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)
    Immunoprecipitation - Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)

    This data was developed using ab271042, the same antibody clone in a different buffer formulation.

    IL-6R was immunoprecipitated from 0.35 mg U266B1 (human multiple myeloma B lymphocyte) whole cell lysate 10 ug with ab271042 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271042 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: U266B1 (human multiple myeloma B lymphocyte) whole cell lysate 10 ug

    Lane 2: ab271042 IP in U266B1 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab271042 in U266B1 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5.5 seconds

  • Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)
    Anti-IL-6R antibody [EPR24322-143] - BSA and Azide free (ab280573)

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