Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23876-52] to Chromogranin C/SGII - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB, IHC-Fr, ICC
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free
    See all Chromogranin C/SGII primary antibodies

  • Description

    Rabbit monoclonal [EPR23876-52] to Chromogranin C/SGII - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Rat
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: SH-SY-5Y, Neuro-2a, Beta-TC-6, GH3 and PC-12 whole cell lysates; Mouse hypothalamus tisue lysate; Rat hypothalamus tissue lysate. IHC-P: Human pancreas tissue; Mouse pancreas tissue; Rat pancreas tissue. IHC-Fr: Mouse pancreas tissue; Rat pancreas tissue. ICC: PC-12 and Beta-TC-6 cells. Flow cyt: PC-12 and Beta-TC-6 cells.

  • General notes

    ab275028 is the carrier-free version of ab275019. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275028 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    All lanes : Anti-Chromogranin C/SGII antibody [EPR23876-52] (ab275019) at 1/1000 dilution

    Lane 1 : Mouse hypothalamus tissue lysate
    Lane 2 : Rat hypothalamus tissue lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 71 kDa



    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer:  5% NFDM/TBST.

    Exposure times: Lane 1: 26 seconds; Lane 2: 48 seconds.

    Chromogranin C/SGII is proteolytically processed to small fragments. 81-kDa, 22 to 48 kDa forms are observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 2282535, 8492910, 16357044, 7883050).

  • Immunocytochemistry - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Immunocytochemistry - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed PC-12 cells labelling Chromogranin C/SGII with ab275019 at 1/50 (10.68 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in pancreatic islet (PMID: 30690892). The section was incubated with ab275019 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Western blot - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Immunocytochemistry - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Beta-TC-6 cells labelling Chromogranin C/SGII with ab275019 at 1/50 (10.68 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Beta-TC-6 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in mouse pancreatic islet (PMID: 30690892). The section was incubated with ab275019 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/500 (1.068 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat pancreatic islet is observed. The nuclear counterstain was DAPI (Blue). Insulin was counterstained using ab6995 at 1/1000 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Flow Cytometry - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Flow Cytometry - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized PC-12 (Rat adrenal gland pheochromocytoma) cells labelling Chromogranin C/SGII with ab275019 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black)  and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Immunohistochemistry (Frozen sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/500 (1.068 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse pancreatic islet is observed. Insulin was counterstained using ab6995 at 1/1000 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (Red).The nuclear counterstain was DAPI (Blue).

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Flow Cytometry - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Flow Cytometry - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Beta-TC-6 (Mouse pancreas insulinoma beta cell) cells labelling Chromogranin C/SGII with ab275019 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

    This data was developed using ab275019, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Chromogranin C/SGII with ab275019 at 1/2000 (0.267 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in human pancreatic islet (PMID: 30690892)The section was incubated with ab275019 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)
    Anti-Chromogranin C/SGII antibody [EPR23876-52] - BSA and Azide free (ab275028)

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