Anti-IL-27-A antibody [EPR18247-101] - BSA and Azide free (ab252238)
![Anti-IL-27-A antibody [EPR18247-101] - BSA and Azide free (ab252238)](https://www.abcam.com/ps/products/252/ab252238/Images/ab252238-341542-831.jpg "Anti-IL-27-A antibody [EPR18247-101] - BSA and Azide free (ab252238)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18247-101] to IL-27-A - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse
Overview
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Product name
Anti-IL-27-A antibody [EPR18247-101] - BSA and Azide free
See all IL-27-A primary antibodies -
Description
Rabbit monoclonal [EPR18247-101] to IL-27-A - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: RAW 264.7 (treated with 1µg/ml LPS for 6h) whole cell lysate.
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General notes
Ab252238 is the carrier-free version of ab190831. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab252238 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18247-101 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-IL-27-A antibody [EPR18247-101] - BSA and Azide free (ab252238)
IL-27-A was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) (treated with 1μg/ml LPS for 6h) whole cell lysate with ab190831 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190831 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: RAW 264.7 (treated with 1μg/ml LPS for 6h ) whole cell lysate 10 μg (Input).
Lane 2: ab190831 IP in RAW 264.7 (treated with 1μg/ml LPS for 6h) whole cell lysate.
Lane 3: : Rabbit monoclonal IgG (ab172730) instead of ab190831 in RAW 264.7 (treated with 1μg/ml LPS for 6h) whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.The molecular weight observed is consistent with what has been described in the literature (PMID: 19620301, PMID: 12121660).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab190831).
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![Anti-IL-27-A antibody [EPR18247-101] - BSA and Azide free (ab252238)](https://www.abcam.com/ps/products/252/ab252238/Images/ab252238-2-benefits-of-recombinant-antibodies.png)
Anti-IL-27-A antibody [EPR18247-101] - BSA and Azide free (ab252238)