Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸ 10 µl 107 212 ₸

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Anti-IL-22 antibody [EPR20211-49] (ab227033)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20211-49] to IL-22
Suitable for: IP, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-IL-22 antibody [EPR20211-49]
See all IL-22 primary antibodies
Description

Rabbit monoclonal [EPR20211-49] to IL-22
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- ICC/IF: 293T cells. Flow Cyt: 293T and Th17 polarized human PBMC treated with 50ng/ml PMA cells. IP: 293T cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20211-49
Isotype

IgG1
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-IL-22 antibody [EPR20211-49] (ab227033)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling IL-22 with ab227033 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and staining in 293T cells transfected with myc-tagged IL-22 expression vector. The vector also contains a PDGFR transmembrane domain. is observed. ab223894 Anti-Myc tag antibody [9E10] (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Flow Cytometry - Anti-IL-22 antibody [EPR20211-49] (ab227033)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Th17 polarized human PBMC treated with 50ng/ml PMA (ab120297), 200ng/ml Ionomycin calcium (ab120116) and 300ng/ml BFA (ab193369) for 4h cells labelling IL-22 with ab227033 at 1/500 dilution(Red) compared with a Rabbit monoclonal IgG (ab172730) Left isotype control (left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-IL-22 antibody [EPR20211-49] (ab227033)

IL-22 was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) transfected with myc-tagged IL-22 expression vector whole cell lysate with ab227033 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab227033 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: 293T (human embryonic kidney epithelial cell) transfected with myc-tagged IL-22 expression vector whole cell lysate 10ug

Lane 2: ab227033 IP in 293T transfected with myc-tagged IL-22 expression vector whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227033 in 293T transfected with myc-tagged IL-22 expression vector whole cell lysate.

The vector containing a PDGFR transmembrane domain (~6 kDa). The molecular weight observed is consistent with what has been described in the literature (PMID: 26059044)

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 20 seconds
Flow Cytometry - Anti-IL-22 antibody [EPR20211-49] (ab227033)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (human embryonic kidney epithelial cell) transfected with myc-tagged IL-22 expression vector cells labelling IL-22 with ab227033 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1:2000 dilution was used as the secondary antibody.
Anti-IL-22 antibody [EPR20211-49] (ab227033)

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