Anti-IL-17C antibody (ab77183)
Key features and details
- Rabbit polyclonal to IL-17C
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL-17C antibody
See all IL-17C primary antibodies -
Description
Rabbit polyclonal to IL-17C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human IL-17C aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94887) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: THP1; Raji; K562.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77183 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 27189 Human
- Omim: 604628 Human
- SwissProt: Q9P0M4 Human
- Unigene: 278911 Human
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Alternative names
- CX2 antibody
- Cytokine CX2 antibody
- IL 17C antibody
see all
Images
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Western blot - Anti-IL-17C antibody (ab77183)All lanes : Anti-IL-17C antibody (ab77183) at 1 µg/ml
Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 18 kDa could potentially be the mature form of Human Interleukin-17C.
Datasheets and documents
References (0)
ab77183 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-IL-17C antibody (ab77183)All lanes : Anti-IL-17C antibody (ab77183) at 1 µg/ml
Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
The band observed at 18 kDa could potentially be the mature form of Human Interleukin-17C.
