Anti-IL-15 antibody [EPR22635-214] (ab273625)
![Anti-IL-15 antibody [EPR22635-214] (ab273625)](https://www.abcam.com/ps/products/273/ab273625/Images/ab273625-1-anti-il15-antibody-epr22635214-flow-cytometry-mouse-pbmc.jpg "Anti-IL-15 antibody [EPR22635-214] (ab273625)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22635-214] to IL-15
- Suitable for: Flow Cyt, IP, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-IL-15 antibody [EPR22635-214]
See all IL-15 primary antibodies -
Description
Rabbit monoclonal [EPR22635-214] to IL-15 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseIP MouseHumanWB MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lymph node tissue lysate; J774A.1, RAW264.7 and A20 whole cell lysates. Flow Cyt: Mouse PBMC cell. IP: Human spleen tissue lysate; Mouse spleen tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22635-214 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-IL-15 antibody [EPR22635-214] (ab273625)
Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Mouse PBMC cells labelling IL-15 with ab273625 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625)](https://www.abcam.com/ps/products/273/ab273625/Images/ab273625-395058-anti-il15-antibody-epr22635214-western-blot-j774a1.jpg)
Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625)All lanes : Anti-IL-15 antibody [EPR22635-214] (ab273625) at 1/1000 dilution
Lane 1 : J774A.1 (mouse reticulum cell sarcoma monocyte macrophage), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 37 seconds.
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![Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625)](https://www.abcam.com/ps/products/273/ab273625/Images/ab273625-395057-anti-il15-antibody-epr22635214-western-blot-mouse-lymph-node-tissue-lysate-20-ug.jpg)
Western blot - Anti-IL-15 antibody [EPR22635-214] (ab273625)All lanes : Anti-IL-15 antibody [EPR22635-214] (ab273625) at 1/1000 dilution
Lane 1 : Mouse lymph node tissue lysate
Lane 2 : Mouse heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression tissue: Heart (Database: HPA).
Exposure time: 26 seconds.
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![Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)](https://www.abcam.com/ps/products/273/ab273625/Images/ab273625-3-anti-il15-antibody-epr22635214-immunoprecipitation-mouse-spleen-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)IL-15 was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate with ab273625 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273625 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse spleen tissue lysate 10 ug
Lane 2: ab273625 IP in Mouse spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273625 in Mouse spleen tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)](https://www.abcam.com/ps/products/273/ab273625/Images/ab273625-2-anti-il15-antibody-epr22635214-immunoprecipitation-human-spleen-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-IL-15 antibody [EPR22635-214] (ab273625)IL-15 was immunoprecipitated from 0.35 mg Human spleen tissue lysate with ab273625 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273625 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human spleen tissue lysate 10 ug
Lane 2: ab273625 IP in Human spleen tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273625 in Human spleen tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-IL-15 antibody [EPR22635-214] (ab273625)](https://www.abcam.com/ps/products/273/ab273625/Images/ab273625-6-benefits-of-recombinant-antibodies.png)
Anti-IL-15 antibody [EPR22635-214] (ab273625)