Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-IKZF3 antibody [EPR9342(B)] (ab139408)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR9342(B)] to IKZF3
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-IKZF3 antibody [EPR9342(B)]
See all IKZF3 primary antibodies
Description

Rabbit monoclonal [EPR9342(B)] to IKZF3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Rat Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human IKZF3 aa 1-100 (N terminal). The exact sequence is proprietary.
(Peptide available as ab184024)
Positive control
- Purchase matching WB positive control:Recombinant Human IKZF3 protein
- WB: Recombinant human IKZF3 protein (ab132618); Human thymus, Jurkat, Raji, and Ramos cell lysates. IHC-P: Human and rat spleen tissues and mouse cardiac muscle tissue. ICC/IF: Jurkat cells. Flow Cyt: Ramos cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR9342(B)
Isotype

IgG
Research areas

Western blot - Anti-IKZF3 antibody [EPR9342(B)] (ab139408)

All lanes : Anti-IKZF3 antibody [EPR9342(B)] (ab139408) at 1/20000 dilution (purified)

Lane 1 : Raji (Human Burkitt's lymphoma cell line) cell lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 58 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody [EPR9342(B)] (ab139408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labeling IKZF3 with purified ab139408 at 1/100.

Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-IKZF3 antibody [EPR9342(B)] (ab139408)

Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling IKZF3 (green) with purified ab139408 at 1/100.

Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

Control: Secondary antibody ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
Flow Cytometry - Anti-IKZF3 antibody [EPR9342(B)] (ab139408)

Flow cytometry analysis of Ramos (Human Burkitt's lymphoma cell line cells labeling IKZF3 with purified ab139408 at 1/90 (red).

Cells were fixed with 2% paraformaldehyde. An FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabeled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-IKZF3 antibody [EPR9342(B)] (ab139408)

All lanes : Anti-IKZF3 antibody [EPR9342(B)] (ab139408) at 1/1000 dilution (unpurified)

Lane 1 : Human thymus cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate
Lane 3 : Raji (Human Burkitt's lymphoma cell line) cell lysate
Lane 4 : Ramos (Human Burkitt's lymphoma cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 58 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody [EPR9342(B)] (ab139408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labeling IKZF3 with purified ab139408 at 1/100.

Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody [EPR9342(B)] (ab139408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labeling IKZF3 with purified ab139408 at 1/100.

Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKZF3 antibody [EPR9342(B)] (ab139408)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labeling IKZF3 with unpurified ab139408 at 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-IKZF3 antibody [EPR9342(B)] (ab139408)

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