Anti-IGF1 Receptor antibody (ab39675)
Key features and details
- Rabbit polyclonal to IGF1 Receptor
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IGF1 Receptor antibody
See all IGF1 Receptor primary antibodies -
Description
Rabbit polyclonal to IGF1 Receptor -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total IGF1 Receptor protein. Crossreacts with insulin receptor (P06213) and insulin receptor related protein (P14616). -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
The antiserum was produced against synthesized non phosphopeptide derived from human IGF1 Receptor around the phosphorylation site of tyrosine1161.
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Positive control
- WB: HEK-293 and SK-OV3 cells treated with 10 mU/ml insulin. HeLa cell lysate. IHC: Human breast carcinoma and brain tumor tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IGF1 Receptor antibody (ab39675)Lanes 1-3 : Anti-IGF1 Receptor antibody (ab39675) (Anti-IGF1 Receptor antibody)
Lanes 4-5 : Anti-IGF1 Receptor (phospho Y1161) antibody (ab39398) (Anti-IGF1 Receptor (phospho-Tyr1161) antibody)
Lanes 1 & 5 : 293 cell lysate treated with insulin (10mU/ml)
Lane 2 : SK-OV3 cell lysate
Lane 3 : SK-OV3 cell lysate with Synthesized peptide
Lane 4 : 293 cell lysate
Predicted band size: 155 kDa
Western blot analysis using IGF1 Receptor antibody (ab39675, Lane 1, 2 and 3) and IGF1 Receptor (phospho-Tyr1161) antibody (ab39398, Lane 4 and 5). Lane 1 and 5 treated with insulin (10mU/ml). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF1 Receptor antibody (ab39675)Paraffin-embedded human breast carcinoma tissue stained for IGF1 Receptor using ab39675 in immunohistochemical analysis. The image on the right is blocked with the synthesized peptide.
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Western blot - Anti-IGF1 Receptor antibody (ab39675)Anti-IGF1 Receptor antibody (ab39675) + HeLa cell lysate
Predicted band size: 155 kDa
