Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5098(2)] to IGF1 - BSA and Azide free
  • Suitable for: ELISA, WB
  • Reacts with: Recombinant fragment

Overview

  • Product name

    Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free
    See all IGF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR5098(2)] to IGF1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IGF1 recombinant protein

  • General notes

    ab221958 is the carrier-free version of ab133542 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab221958 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
    Western blot - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
    Anti-IGF1 antibody [EPR5098(2)] (ab133542) at 1/1000 dilution + Recombinant human IGF-I fragment at 0.005 µg

    Predicted band size: 22 kDa



    This data was developed using ab133542, the same antibody clone in a different buffer formulation.

    The recombinant IGF-I fragment corresponds to amino acids 49-118 of human IGF-1 with the molecular weight of 10kDa.

  • ELISA - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
    ELISA - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
    This data was developed using ab133542, the same antibody clone in a different buffer formulation.Primary antibody, ab33542 was used at concentrations between 0.1 - 1000ng/ml. An Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at 1:2500 dilution. Antigen concentration was 1000ng/ml.
  • OI-RD Scanning - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
    OI-RD Scanning - Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
    This data was developed using ab133542, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)
    Anti-IGF1 antibody [EPR5098(2)] - BSA and Azide free (ab221958)

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