Anti-IDH3A antibody (ab228596)
Key features and details
- Rabbit polyclonal to IDH3A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IDH3A antibody
See all IDH3A primary antibodies -
Description
Rabbit polyclonal to IDH3A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Zebrafish, Rhesus monkey -
Immunogen
Recombinant fragment within Human IDH3A (internal sequence). The exact sequence is proprietary.
Database link: P50213 -
Positive control
- WB: Mouse and rat brain tissue lysate: HepG2 whole cell lysate (ab7900). IHC-P: Human lung SCC tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-IDH3A antibody (ab228596) at 1/10000 dilution + Rat brain tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 40 kDa10% SDS-PAGE
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for IDH3A (green) using ab228596 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.
Blue: Hoechst 33342 staining.
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Paraffin-embedded human lung SCC tissue stained for IDH3A with ab228596 at 1/100 dilution in immunohistochemical analysis
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Anti-IDH3A antibody (ab228596) at 1/10000 dilution + Mouse brain tissue lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 40 kDa10% SDS-PAGE
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Anti-IDH3A antibody (ab228596) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 40 kDa12% SDS-PAGE