Anti-HYAL1 antibody (ab96751)
Key features and details
- Rabbit polyclonal to HYAL1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HYAL1 antibody
See all HYAL1 primary antibodies -
Description
Rabbit polyclonal to HYAL1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human HYAL1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab107788) -
Positive control
- This antibody gave a positive signal in the following Human tissue lysates: Liver; Kidney; Heart; Breast.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 48 kDa).Target
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Function
May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth. -
Tissue specificity
Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues. -
Involvement in disease
Defects in HYAL1 are the cause of mucopolysaccharidosis type 9 (MPS9) [MIM:601492]; also called hyaluronidase deficiency. MPS9 is a lysosomal storage disease characterized by high hyaluronan (HA) concentration in the serum. The clinical features are periarticular soft tissue masses, mild short stature and acetabular erosions, and absence of neurological or visceral involvement. -
Sequence similarities
Belongs to the glycosyl hydrolase 56 family.
Contains 1 EGF-like domain. -
Cellular localization
Secreted. Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 3373 Human
- Omim: 607071 Human
- SwissProt: Q12794 Human
- Unigene: 75619 Human
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Alternative names
- HYAL 1 antibody
- Hyal-1 antibody
- HYAL1 antibody
see all
Images
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Western blot - Anti-HYAL1 antibody (ab96751)All lanes : Anti-HYAL1 antibody (ab96751) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Human breast tissue lysate - total protein (ab30090)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
The band observed at 45 kDa could potentially be a cleaved form of HYAL1 due to the presence of a 21 amino acid signal peptide.
Datasheets and documents
References (0)
ab96751 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-HYAL1 antibody (ab96751)All lanes : Anti-HYAL1 antibody (ab96751) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Human breast tissue lysate - total protein (ab30090)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 48 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
The band observed at 45 kDa could potentially be a cleaved form of HYAL1 due to the presence of a 21 amino acid signal peptide.
