Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸

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Anti-Human IgM antibody [EPR20731] (ab212201)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20731] to Human IgM
Suitable for: WB, IP, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-Human IgM antibody [EPR20731]
See all Human IgM primary antibodies
Description

Rabbit monoclonal [EPR20731] to Human IgM
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Raji whole cell lysate; human tonsil and fetal spleen tissue lysates. IP: Raji whole cell lysate. ICC/IF: Raji cells. Flow cytometry: Human PBMC cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20731
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Human IgM antibody [EPR20731] (ab212201)

Immunofluorescent analysis of 100% methanol-fixed Raji (human Burkitt's lymphoma cell line) cells labeling IgM with ab212201 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in Raji cells.

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^®594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-Human IgM antibody [EPR20731] (ab212201)

All lanes : Anti-Human IgM antibody [EPR20731] (ab212201) at 1/1000 dilution

Lane 1 : Raji (human Burkitt's lymphoma cell line) whole cell lysate at 20 µg
Lane 2 : Human tonsil tissue lysate at 20 µg
Lane 3 : Human fetal spleen tissue lysate at 10 µg

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Developed using the ECL technique.

Predicted band size: 49 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
The molecular mass observed is consistent with the literature (PMID 16199094; PMID: 15955802; PMID 2107027).

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1 & 3: 3 minutes.

Lane 2: 10 seconds.
Flow Cytometry - Anti-Human IgM antibody [EPR20731] (ab212201)

Flow cytometric analysis of human PBMC (peripheral blood mononuclear cells) cell line labeling IgM with ab212201 at 1/50 (right panel) compared with an isotype control details (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Human PBMCs co-stained with anti-CD19 (Y-axis); only CD19+ population give a positive signal for IgM. Gated on total viable cells.
Immunoprecipitation - Anti-Human IgM antibody [EPR20731] (ab212201)

IgM was immunoprecipitated from 0.35 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with ab212201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab212201 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1: Raji whole cell lysate 10 μg (Input).

Lane 2: ab212201 IP in Raji whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab212201 in Raji whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds.
Anti-Human IgM antibody [EPR20731] (ab212201)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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