Anti-Mouse IgM antibody [RM109] (ab190369)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM109] to Mouse IgM
- Suitable for: ELISA
- Reacts with: Mouse, Recombinant fragment
Overview
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Product name
Anti-Mouse IgM antibody [RM109] -
Description
Rabbit monoclonal [RM109] to Mouse IgM -
Host species
Rabbit -
Specificity
This antibody reacts to mouse IgM. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The Fc region of RM109 has been engineered to eliminate the binding of Fc receptor. -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragment -
Immunogen
Full length protein corresponding to Mouse IgM.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab190369 was purified from an animal origin–free and protein-free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM109 -
Isotype
IgG -
Research areas
Images
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A titer ELISA of mouse IgM. The plate was coated with different amounts of mouse IgM. A serial dilution of ab190369 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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ELISA of mouse immunoglobulins shows ab190369 reacted to mouse IgM; no cross reactivity with IgG1, IgG2a, IgG2b, IgG3, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab190369 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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