Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)
![Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)](https://www.abcam.com/ps/products/6/ab6514/Images/ab6514-280676-anti-hsv-1-icp4-immediate-early-protein-antibody-10f1-western-blot.jpg "Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)")
Key features and details
- Mouse monoclonal [10F1] to HSV 1 ICP4 Immediate Early Protein
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] -
Description
Mouse monoclonal [10F1] to HSV 1 ICP4 Immediate Early Protein -
Host species
Mouse -
Specificity
ELISA titer 1/25,600 absorption of 0.10 -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Herpes Virus
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
10F1 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)Lane 1 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/200 dilution
Lane 2 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/400 dilution
Lane 3 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/800 dilution
Lane 4 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1600 dilution
All lanes : BCIP/NBT. HSV-1 MacIntyre/Vero infected cell extract
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Mouse secondary at 1/5000 dilution
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![Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)](https://www.abcam.com/ps/products/6/ab6514/Images/ab6514-12353-anti-hsv-1-icp4-immediate-early-protein-antibody-10f1-western-blot.jpg)
Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) This image is courtesy of an anonymous AbreviewAll lanes : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1000 dilution
Lane 1 : HeLa cells (whole cell lysate) - mock infection, cytosolic fraction
Lane 2 : HeLa cells (whole cell lysate) - mock infection, membrane fraction
Lane 3 : HeLa cells (whole cell lysate) - mock infection, nuclear fraction
Lane 4 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - cytosolic fraction
Lane 5 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours -membrane fraction
Lane 6 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - nuclear fraction
Lane 7 : HeLa cells (whole cell lysate) - mock infected
Lane 8 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated sheep anti-mouse IgG
Observed band size: 97 kDa why is the actual band size different from the predicted?
As shown, ICP4 is detected mainly in the nuclear fraction of infected cells (lane 6). The presence of ICP4 in the cytosolic fraction (lane 4) probably represents the protein undergoing synthesis. -
![Immunocytochemistry/ Immunofluorescence - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514)](https://www.abcam.com/ps/products/6/ab6514/Images/ab6514-12354-anti-hsv-1-icp4-immediate-early-protein-antibody-10f1-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) This image is courtesy of an anonymous Abreviewab6514 (1/5000) staining HSV1 ICP4 immediate Early Protein in mouse 293T cells transiently transfected with a plasmid expressing HSV ICP4 (green). Cells were formaldehyde-fixed and incubated with the primary antibody for 1 hour. A FITC conjugated rabbit polyclonal antibody was used as the secondary antibody.