Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I
- Suitable for: WB, ICC, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Cannabinoid Receptor I antibody [EPR23934-20]
See all Cannabinoid Receptor I primary antibodies -
Description
Rabbit monoclonal [EPR23934-20] to Cannabinoid Receptor I -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC MouseRatHumanWB MouseRatHuman -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain and hippocampus tissue lysate; Mouse brain tissue lysate; Rat brain tissue lysate; SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate; NIH/3T3 (mouse embryonic fibroblast) whole cell lysate. ICC: SH-SY5Y cells; mouse primary neuron; rat primary neuron. Flow Cyt: SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23934-20 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human hippocampus tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 53 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1: 3 minutes; Lanes 2-4: 70 seconds.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y cells labelling Cannabinoid receptor 1 with ab259323 at 1/100 (5.44 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SH-SY5Y cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 53 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: Lane 1: 67 seconds; Lane 2: 3 minutes.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
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All lanes : Anti-Cannabinoid Receptor I antibody [EPR23934-20] (ab259323) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse liver tissue lysate
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 53 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 9655502).
Negative control: mouse liver (PMID: 9655502).
Exposure time: 70 seconds
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neuron cells labelling Cannabinoid receptor 1 with ab259323 at 1/500 (1.088 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection is observed. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.