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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins

Anti-Hsp90 beta (phospho S226) antibody (ab63562)

Anti-Hsp90 beta (phospho S226) antibody (ab63562)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to Hsp90 beta (phospho S226)
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-Hsp90 beta (phospho S226) antibody
    See all Hsp90 beta primary antibodies
  • Description

    Rabbit polyclonal to Hsp90 beta (phospho S226)
  • Host species

    Rabbit
  • Specificity

    Detects Hsp90 beta only when phosphorylated at serine 226.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic phosphopeptide derived from human Hsp90 beta around the phosphorylation site of serine 226 (E-I-SP-D-D).

  • Positive control

    • NIH/3T3 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins

Images

  • Western blot - Anti-Hsp90 beta (phospho S226) antibody (ab63562)
    Western blot - Anti-Hsp90 beta (phospho S226) antibody (ab63562)
    All lanes : Anti-Hsp90 beta (phospho S226) antibody (ab63562) at 1/500 dilution

    Lane 1 : extracts from A549 cells, treated
    with serum (20%, 15mins)
    Lane 2 : extracts from A549 cells, treated
    with serum (20%, 15mins) with immunizing phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta (phospho S226) antibody (ab63562)
    Immunocytochemistry/ Immunofluorescence - Anti-Hsp90 beta (phospho S226) antibody (ab63562)
    ab63562 at 1/500 dilution staining NIH/3T3 cells, without (left) and with (right) immunizing peptide.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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