Anti-Hsp90 beta antibody [K3705] (ab82522)
![Anti-Hsp90 beta antibody [K3705] (ab82522)](https://www.abcam.com/ps/products/82/ab82522/Images/ab82522-388317-anti-hsp90-beta-antibody-k3705-western-blot-a431-wt-a431-hsp90ab1-knockout-saos2-hl60.jpg "Anti-Hsp90 beta antibody [K3705] (ab82522)")
Key features and details
- Mouse monoclonal [K3705] to Hsp90 beta
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgM
Overview
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Product name
Anti-Hsp90 beta antibody [K3705]
See all Hsp90 beta primary antibodies -
Description
Mouse monoclonal [K3705] to Hsp90 beta -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseRatHumanChinese hamster
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Immunogen
Recombinant human Hsp90 beta protein
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Positive control
- WB: A431, Saos-2, HL-60, HeLa, PC-12 cells, CHO-K1 cells and 3T3 cells. IHC-P: Human pancreas FFPE tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.90
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Clonality
Monoclonal -
Clone number
K3705 -
Isotype
IgM -
Research areas
Images
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Western blot - Anti-Hsp90 beta antibody [K3705] (ab82522)All lanes : Anti-Hsp90 beta antibody [K3705] (ab82522) at 1/1000 dilution
Lane 1 : Wild-type A431 cell lysate
Lane 2 : HSP90AB1 knockout A431 cell lysate
Lane 3 : Saos-2 cell lysate
Lane 4 : HL-60 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab82522 observed at 85 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab82522 was shown to react with Hsp90 beta in wild-type A431 cells in western blot. Loss of signal was observed when HSP90AB1 knockout sample was used. Wild-type A431 and HSP90AB1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab82522 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Hsp90 beta antibody [K3705] (ab82522)](https://www.abcam.com/ps/products/82/ab82522/Images/ab82522-97311-anti-hsp90-beta-antibody-k3705-western-blot.jpg)
Western blot - Anti-Hsp90 beta antibody [K3705] (ab82522)All lanes : Anti-Hsp90 beta antibody [K3705] (ab82522) at 1/1000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Hsp90
Native Human Protein
Lane 3 : Hsp90 alpha Recombinant Human
Protein
Lane 4 : Hsp90 beta Recombinant Human Protein
Lane 5 : HeLa cells
Lane 6 : PC-12 cells
Lane 7 : CHO-K1 cells
Lane 8 : 3T3 cells
Predicted band size: 83 kDa
Observed band size: 83 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [K3705] (ab82522)](https://www.abcam.com/ps/products/82/ab82522/Images/Hsp90-beta-Primary-antibodies-ab82522-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsp90 beta antibody [K3705] (ab82522)ab82522, at 1/50 dilution, staining Hsp90 beta in human breast cancer tissue by Immunohistochemistry.
