Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1712] to HSF1 (phospho S320) - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free
    See all HSF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1712] to HSF1 (phospho S320) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247451 is the carrier-free version of ab76183 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247451 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
    This data was developed using ab76183, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human muscles using ab76183 at 1/100 dilution. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Western blot - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
    Western blot - Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
    All lanes : Anti-HSF1 (phospho S320) antibody [EPR1712] (ab76183) at 1/20000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HeLa cell lysate, heat treated at 42°C

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit-HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 57 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab76183, the same antibody clone in a different buffer formulation.

     

  • Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)
    Anti-HSF1 (phospho S320) antibody [EPR1712] - BSA and Azide free (ab247451)

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