Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HSF1 antibody [EPR23673-128] (ab242138)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23673-128] to HSF1
  • Suitable for: WB, ICC, Flow Cyt
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-HSF1 antibody [EPR23673-128]
    See all HSF1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23673-128] to HSF1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    ICC
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse ovary and testis tissue lysates; Rat testis tissue lysate; NIH/3T3 and PC-12 whole cell lysates. ICC: NIH/3T3 and C2C12 cells. Flow cyt: NIH/3T3 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HSF1 antibody [EPR23673-128] (ab242138)
    Western blot - Anti-HSF1 antibody [EPR23673-128] (ab242138)
    All lanes : Anti-HSF1 antibody [EPR23673-128] (ab242138) at 1/1000 dilution

    Lane 1 : Mouse ovary tissue lysate
    Lane 2 : Mouse testis tissue lysate
    Lane 3 : Rat testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 57 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10747023, 28194040, 27474884).

    Exposure time: 3 minutes.

     

  • Flow Cytometry - Anti-HSF1 antibody [EPR23673-128] (ab242138)
    Flow Cytometry - Anti-HSF1 antibody [EPR23673-128] (ab242138)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labelling HSF1 with ab242138 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry - Anti-HSF1 antibody [EPR23673-128] (ab242138)
    Immunocytochemistry - Anti-HSF1 antibody [EPR23673-128] (ab242138)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 cells labelling HSF1 with ab242138 at 1/50 (9.4 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in NIH/3T3 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-HSF1 antibody [EPR23673-128] (ab242138)
    Western blot - Anti-HSF1 antibody [EPR23673-128] (ab242138)
    All lanes : Anti-HSF1 antibody [EPR23673-128] (ab242138) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 57 kDa
    Observed band size: 80 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Lysates were made freshly and used in WB test immediately to minimize protein degradation.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10747023, 28194040, 27474884).

     Exposure times: Lane 1: 15 seconds; Lane 2: 37 seconds.

     

  • Immunocytochemistry - Anti-HSF1 antibody [EPR23673-128] (ab242138)
    Immunocytochemistry - Anti-HSF1 antibody [EPR23673-128] (ab242138)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling HSF1 with ab242138 at 1/50 (9.4 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in C2C12 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Anti-HSF1 antibody [EPR23673-128] (ab242138)
    Anti-HSF1 antibody [EPR23673-128] (ab242138)

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