Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HRAS antibody [M337] (ab86696)

Key features and details

  • Mouse monoclonal [M337] to HRAS
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-HRAS antibody [M337]

  • Description

    Mouse monoclonal [M337] to HRAS

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat

  • Immunogen

    Recombinant full length protein corresponding to Human HRAS.

  • Positive control

    • Adult mouse brain, human A431 cell and human Jurkat cell lysates.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-HRAS antibody [M337] (ab86696)
    Western blot - Anti-HRAS antibody [M337] (ab86696)
    All lanes : Anti-HRAS antibody [M337] (ab86696) at 1/500 dilution

    Lane 1 : Mouse brain
    Lane 2 : Human A431 cell lysate
    Lane 3 : Jurkat cell lysate

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRAS antibody [M337] (ab86696)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HRAS antibody [M337] (ab86696)
    IHC image of ab86696 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab86696, undiluted, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • Western blot - Anti-HRAS antibody [M337] (ab86696)
    Western blot - Anti-HRAS antibody [M337] (ab86696) Data courtesy of an anonymous collaborator
    All lanes : Anti-HRAS antibody [M337] (ab86696)

    Lane 1 : RASless MEFs overexpressing Human BRAF
    Lane 2 : RASless MEFs overexpressing Human HRAS
    Lane 3 : RASless MEFs overexpressing Human NRAS
    Lane 4 : RASless MEFs overexpressing Human KRAS

    Predicted band size: 21 kDa

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