Anti-HPD antibody - N-terminal (ab188436)
Key features and details
- Rabbit polyclonal to HPD - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HPD antibody - N-terminal
See all HPD primary antibodies -
Description
Rabbit polyclonal to HPD - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human HPD aa 1-100 (N terminal).
Database link: P32754 -
Positive control
- IHC-P: Human kidney and liver tissues. WB: Liver tissue lysate and HepG2 cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-HPD antibody - N-terminal (ab188436) at 0.4 µg/ml
Lane 1 : Liver tissue lysate
Lane 2 : HepG2 cell lysate
Predicted band size: 45 kDa
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Paraffin embedded human kidney tissue stained for HPD using ab188436 at 1/50 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling HPD with ab188436 at 1/20 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Paraffin embedded human squamous epithelia tissue stained for HPD using ab188436 at 1/50 dilution in immunohistochemical analysis. Shows no positivity in squamous epithelial cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Paraffin embedded human endometrium tissue stained for HPD using ab188436 at 1/50 dilution in immunohistochemical analysis. Shows no positivity in squamous epithelial cells as expected.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.