Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HPD antibody [EPR5297] (ab133515)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5297] to HPD
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-HPD antibody [EPR5297]
    See all HPD primary antibodies

  • Description

    Rabbit monoclonal [EPR5297] to HPD

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to residues in Human HPD (UniProt ID: P32754)

  • Positive control

    • HepG2 and Fetal liver lysates. Human kidney tissue.

  • General notes

     

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-HPD antibody [EPR5297] (ab133515)
    Western blot - Anti-HPD antibody [EPR5297] (ab133515)
    Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling HPD with purified ab133515 at 1:2000 dilution (0.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-HPD antibody [EPR5297] (ab133515)
    Western blot - Anti-HPD antibody [EPR5297] (ab133515)
    Anti-HPD antibody [EPR5297] (ab133515) at 1/5000 dilution (purified) + Rat liver lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-HPD antibody [EPR5297] (ab133515)
    Western blot - Anti-HPD antibody [EPR5297] (ab133515)
    All lanes : Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 lysate
    Lane 2 : Fetal liver lysate
    Lane 3 : Human kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] (ab133515)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HPD with unpurified ab133515 at 1/250 dilution.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-HPD antibody [EPR5297] (ab133515)
    OI-RD Scanning - Anti-HPD antibody [EPR5297] (ab133515)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-HPD antibody [EPR5297] (ab133515)
    Anti-HPD antibody [EPR5297] (ab133515)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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