Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5297] to HPD - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-HPD antibody [EPR5297] - BSA and Azide free
    See all HPD primary antibodies

  • Description

    Rabbit monoclonal [EPR5297] to HPD - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248533 is the carrier-free version of ab133515 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248533 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    Western blot - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution (purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa



    This data was developed using ab133515, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    This data was developed using ab133515, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling HPD with purified ab133515 at 1:2000 dilution (0.76 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    Western blot - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    Anti-HPD antibody [EPR5297] (ab133515) at 1/5000 dilution (purified) + Rat liver tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa



    This data was developed using ab133515, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    Western blot - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    All lanes : Anti-HPD antibody [EPR5297] (ab133515) at 1/1000 dilution (unpurified)

    Lane 1 : HepG2 cell lysate
    Lane 2 : Fetal liver tissue lysate
    Lane 3 : Human kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 45 kDa



    This data was developed using ab133515, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    This data was developed using ab133515, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling HPD with unpurified ab133515 at 1/250 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    OI-RD Scanning - Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    This data was developed using ab133515, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)
    Anti-HPD antibody [EPR5297] - BSA and Azide free (ab248533)

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