Anti-HOXA4 antibody (ab26097)
Key features and details
- Rabbit polyclonal to HOXA4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HOXA4 antibody
See all HOXA4 primary antibodies -
Description
Rabbit polyclonal to HOXA4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human HOXA4.
Read Abcam's proprietary immunogen policy (Peptide available as ab32311.) -
Positive control
- HESCS differentiated neuroectoderm lysate at day 10; HESCS differentiated neuroectoderm lysate at day 30.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab26097 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 37-39 kDa (predicted molecular weight: 34 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37-39 kDa (predicted molecular weight: 34 kDa).Application notesIs unsuitable for ICC/IF or IHC-P.Target
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Function
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'. -
Tissue specificity
Embryonic nervous system. -
Sequence similarities
Belongs to the Antp homeobox family. Deformed subfamily.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3201 Human
- Entrez Gene: 15401 Mouse
- Omim: 142953 Human
- SwissProt: Q00056 Human
- SwissProt: P06798 Mouse
- Unigene: 533357 Human
- Unigene: 721126 Human
- Unigene: 439647 Mouse
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Alternative names
- Dfd like protein antibody
- Homeo box A4 antibody
- Homeobox A4 antibody
see all
Images
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Western blot - Anti-HOXA4 antibody (ab26097)Lane 1 : Marker
Lanes 2-4 : Anti-HOXA4 antibody (ab26097) at 1 µg/ml
Lane 2 : Neuroectoderm (HESCS differentiated) Lysate - Day 10 at 20 µg
Lane 3 : Neuroectoderm (HESCS differentiated) Lysate - Day 30 at 20 µg
Lane 4 : Human Skeletal Muscle Normal Tissue Lysate at 20 µg
Secondary
Lanes 2-4 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Higher migrating bands observed in Neuroectoderm (HESCS differentiated) Lysate - Day 10 may represent splice variants of HoxA4. The immunogen is specific for HoxA4 in NCBI blast and shows no similarity to other HOX proteins.
Datasheets and documents
References (5)
ab26097 has been referenced in 5 publications.
- Kimura M et al. Homeobox A4 suppresses vascular remodeling by repressing YAP/TEAD transcriptional activity. EMBO Rep 21:e48389 (2020). PubMed: 32147946
- Klausen C & Auersperg N HOXA4 protein levels and localization in the aorta and in human abdominal aortic aneurysms. BMC Physiol 11:18 (2011). WB ; Human . PubMed: 22168796
- Lillvis JH et al. Regional expression of HOXA4 along the aorta and its potential role in human abdominal aortic aneurysms. BMC Physiol 11:9 (2011). WB, IHC . PubMed: 21627813
- Ota T et al. Expression and function of HOXA genes in normal and neoplastic ovarian epithelial cells. Differentiation 77:162-71 (2009). PubMed: 19281776
- Klausen C et al. Cell motility and spreading are suppressed by HOXA4 in ovarian cancer cells: possible involvement of beta1 integrin. Mol Cancer Res 7:1425-37 (2009). PubMed: 19723874
Images
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Western blot - Anti-HOXA4 antibody (ab26097)Lane 1 : Marker
Lanes 2-4 : Anti-HOXA4 antibody (ab26097) at 1 µg/ml
Lane 2 : Neuroectoderm (HESCS differentiated) Lysate - Day 10 at 20 µg
Lane 3 : Neuroectoderm (HESCS differentiated) Lysate - Day 30 at 20 µg
Lane 4 : Human Skeletal Muscle Normal Tissue Lysate at 20 µg
Secondary
Lanes 2-4 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 34 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?Higher migrating bands observed in Neuroectoderm (HESCS differentiated) Lysate - Day 10 may represent splice variants of HoxA4. The immunogen is specific for HoxA4 in NCBI blast and shows no similarity to other HOX proteins.
