Anti-Homeobox protein SIX6 antibody (ab251658)
Key features and details
- Rabbit polyclonal to Homeobox protein SIX6
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Homeobox protein SIX6 antibody -
Description
Rabbit polyclonal to Homeobox protein SIX6 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken
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Immunogen
Recombinant fragment corresponding to Human Homeobox protein SIX6 aa 50 to the C-terminus.
Database link: O95475 -
Positive control
- IHC-P: Human pituitary gland tissue. ICC/IF: SH-SY5Y cells. WB: Human eye tissue lysate; HEK-293T overexpressing Homeobox protein SIX6 with C-terminal myc-DDK tag, whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Homeobox protein SIX6 antibody (ab251658)PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for Homeobox protein SIX6 (green) using ab251658 at 4 µg/ml in ICC/IF.
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Western blot - Anti-Homeobox protein SIX6 antibody (ab251658)Anti-Homeobox protein SIX6 antibody (ab251658) at 0.4 µg/ml + Human eye tissue lysate
Predicted band size: 28 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein SIX6 antibody (ab251658)Formalin-fixed, paraffin-embedded human retina tissue stained for SIX6 with ab251658 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homeobox protein SIX6 antibody (ab251658)Formalin-fixed, paraffin-embedded human retina pituitary gland stained for SIX6 with ab251658 at a 1/200 dilution in immunohistochemical analysis.
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Western blot - Anti-Homeobox protein SIX6 antibody (ab251658)All lanes : Anti-Homeobox protein SIX6 antibody (ab251658) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T overexpressing Homeobox protein SIX6 with C-terminal myc-DDK tag, whole cell lysate
Predicted band size: 28 kDa