Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12278(2)(B)] to hnRNP U/p120 - BSA and Azide free
  • Suitable for: IHC-P, ICC, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free
    See all hnRNP U/p120 primary antibodies

  • Description

    Rabbit monoclonal [EPR12278(2)(B)] to hnRNP U/p120 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250283 is the carrier-free version of ab180952. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250283 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12278(2)(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
    Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
    All lanes : Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] (ab180952) at 1/20000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 91 kDa



    This data was developed using ab180952, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
    This data was developed using ab180952, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney labeling hnRNP U/p120 using ab180952 at a 1/100 dilution, with Hematoxylin counter staining. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
    Immunocytochemistry - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
    This data was developed using ab180952, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of -20°C acetone-fixed Jurkat cells labeling hnRNP U/p120 using ab180952 at a 1/100 dilution (green) with Dapi counter staining (blue). Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody.
  • Flow Cytometry - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
    Flow Cytometry - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
    This data was developed using ab180952, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling hnRNP U/p120 using ab180952 at a 1/100 dilution, or Rabbit monoclonal IgG as an isotype control.
  • Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
    Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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