Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
![Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)](https://www.abcam.com/ps/products/250/ab250283/Images/ab250283-408404-anti-hnrnp-u-p120-antibody-epr12278-2-b-bsa-and-azide-free-western-blot.jpg "Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12278(2)(B)] to hnRNP U/p120 - BSA and Azide free
- Suitable for: IHC-P, ICC, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free
See all hnRNP U/p120 primary antibodies -
Description
Rabbit monoclonal [EPR12278(2)(B)] to hnRNP U/p120 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250283 is the carrier-free version of ab180952. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250283 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR12278(2)(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)All lanes : Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] (ab180952) at 1/20000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 91 kDaThis data was developed using ab180952, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)](https://www.abcam.com/ps/products/250/ab250283/Images/ab250283-3-anti-hnrnp-u-p120-antibody-epr12278-2-b-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)This data was developed using ab180952, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney labeling hnRNP U/p120 using ab180952 at a 1/100 dilution, with Hematoxylin counter staining. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Immunocytochemistry - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)](https://www.abcam.com/ps/products/250/ab250283/Images/ab250283-2-anti-hnrnp-u-p120-antibody-epr12278-2-b-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)This data was developed using ab180952, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of -20°C acetone-fixed Jurkat cells labeling hnRNP U/p120 using ab180952 at a 1/100 dilution (green) with Dapi counter staining (blue). Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody. -
![Flow Cytometry - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)](https://www.abcam.com/ps/products/250/ab250283/Images/ab250283-1-anti-hnrnp-u-p120-antibody-epr12278-2-b-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283) -
![Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)](https://www.abcam.com/ps/products/250/ab250283/Images/ab250283-5-benefits-of-recombinant-antibodies.png)
Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] - BSA and Azide free (ab250283)
