Anti-HMGCS2 antibody (ab157225)
Key features and details
- Rabbit polyclonal to HMGCS2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HMGCS2 antibody
See all HMGCS2 primary antibodies -
Description
Rabbit polyclonal to HMGCS2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human HMGCS2 aa 450 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab157391) -
Positive control
- This antibody gave a positive signal in Human Liver tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications ChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB 1/250. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Notes WB
1/250. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Target
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Function
This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase. -
Tissue specificity
High expression in liver and colon. Low expression in testis, heart, skeletal muscle and kidney. -
Pathway
Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3. -
Involvement in disease
Defects in HMGCS2 are the cause of HMG-CoA synthase deficiency (HMGCS deficiency) [MIM:605911]; also known as deficiency of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2. Affected individuals present with severe hypoketotic hypoglycemia, mild hepatomegaly, or fatty liver, and a nondiagnostic pattern of urinary organic acids with increase of medium and short chain dicarboxylic acids. -
Sequence similarities
Belongs to the HMG-CoA synthase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 3158 Human
- Omim: 600234 Human
- SwissProt: P54868 Human
- Unigene: 59889 Human
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Alternative names
- 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) antibody
- 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 antibody
- 3 hydroxy 3 methylglutaryl coenzyme A synthase antibody
see all
Images
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Western blot - Anti-HMGCS2 antibody (ab157225)Anti-HMGCS2 antibody (ab157225) at 1/250 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab157225 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab157225 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-HMGCS2 antibody (ab157225)Anti-HMGCS2 antibody (ab157225) at 1/250 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab157225 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
