Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HMGCS2 antibody [EPR8642] (ab137043)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8642] to HMGCS2
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-HMGCS2 antibody [EPR8642]
    See all HMGCS2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8642] to HMGCS2

  • Host species

    Rabbit

  • Specificity

    Does not cross react with HMGCS1.

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human HMGCS2 aa 450 to the C-terminus.
    Database link: P54868

  • Positive control

    • WB: Human fetal liver, mouse kidney and rat kidney tissue lysates and Caco 2 cell lysate. IHC-P: Human, mouse and rat liver tissues and human colon tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/10000 dilution (purified) + Human fetal liver tissue lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    All lanes : Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/5000 dilution (purified)

    Lane 1 : Caco-2 whole cell lysate
    Lane 2 : Mouse kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Western blot - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    All lanes : Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal liver lysate
    Lane 2 : Caco 2 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 57 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling HMGCS2 with unpurified ab137043 at a diltuion of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGCS2 antibody [EPR8642] (ab137043)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCS2 with unpurified ab137043 at a dilution of 1/50.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-HMGCS2 antibody [EPR8642] (ab137043)
    Anti-HMGCS2 antibody [EPR8642] (ab137043)

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